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SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated <t>lncRNA-related</t> mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001
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SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated <t>lncRNA-related</t> mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001
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SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated <t>lncRNA-related</t> mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001
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SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated <t>lncRNA-related</t> mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001
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SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated <t>lncRNA-related</t> mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001
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lncrna/mrna expression profile microarray  (Arraystar inc)
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SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated <t>lncRNA-related</t> mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001
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12x135k human lncrna expression microarray  (Arraystar inc)
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SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated <t>lncRNA-related</t> mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001
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human circrna array v2.0  (Arraystar inc)
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Functional analysis of the cceGPs. A) The 18 cceGPs or gene <t>pair-circRNA</t> motifs in SOC. B) Heatmap illustrating the expression abundance of circRNAs. C) Enriched functions of the 18 cceGPs in Reactome. D) Enriched functions of the 18 cceGPs in GO. Yellow node denotes functional category while grey node represents gene. E, F) Expression abundance of PRC1 and BBS4 in normal controls and SOCs. G, H) Correlations between circHUNK and PRC1 (BBS4 ) in SOCs. I) PRC1 -circHUNK- BBS4 as an example of cceGPs relationship in SOC progress.
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SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated lncRNA-related mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: M 6 A -mediated lncRNA SCIRT stability promotes NSCLC progression through binding to SFPQ and activating the PI3K/Akt pathway

doi: 10.1007/s00018-025-05594-z

Figure Lengend Snippet: SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated lncRNA-related mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001

Article Snippet: Arraystar m 6 A lncRNA epitranscriptomic microarrays were used to screen differentially m 6 A-methylated lncRNAs in NSCLC, and array figures were shown in Supplementary Fig. . A total of 39 differential metylated candidates were identified with 24 upregulated genes and 15 downregulated genes (Table ).

Techniques: Modification, Expressing, Methylation

Functional analysis of the cceGPs. A) The 18 cceGPs or gene pair-circRNA motifs in SOC. B) Heatmap illustrating the expression abundance of circRNAs. C) Enriched functions of the 18 cceGPs in Reactome. D) Enriched functions of the 18 cceGPs in GO. Yellow node denotes functional category while grey node represents gene. E, F) Expression abundance of PRC1 and BBS4 in normal controls and SOCs. G, H) Correlations between circHUNK and PRC1 (BBS4 ) in SOCs. I) PRC1 -circHUNK- BBS4 as an example of cceGPs relationship in SOC progress.

Journal: bioRxiv

Article Title: Noncoding RNA’s competing endogenous gene pair as motif in serous ovarian cancer

doi: 10.1101/2022.04.04.486923

Figure Lengend Snippet: Functional analysis of the cceGPs. A) The 18 cceGPs or gene pair-circRNA motifs in SOC. B) Heatmap illustrating the expression abundance of circRNAs. C) Enriched functions of the 18 cceGPs in Reactome. D) Enriched functions of the 18 cceGPs in GO. Yellow node denotes functional category while grey node represents gene. E, F) Expression abundance of PRC1 and BBS4 in normal controls and SOCs. G, H) Correlations between circHUNK and PRC1 (BBS4 ) in SOCs. I) PRC1 -circHUNK- BBS4 as an example of cceGPs relationship in SOC progress.

Article Snippet: We performed Arraystar Human LncRNA Microarray V2.0 and Arraystar Human circRNA Array V2.0 analyses on all 16 samples.

Techniques: Functional Assay, Expressing